Abstract: Walleye Sander vitreus is the most important exploited Lake Erie fish, whose genetic stock structure is important to resource management. Past research shows that the genetic composition of walleye spawning in Lake Erie tributary and reef sites significantly differs. Using high-resolution nuclear microsatellite markers, the present study is the first to test whether the genetic composition of walleye spawning population groups is temporally stable. We employ 15 nuclear microsatellite loci to test the genetic structure of ~1500 walleye spawning in the Maumee, Sandusky, Grand (OH), and Grand (ON) Rivers, Van Buren Bay, Cattaraugus Creek, and Lake St. Clair across 14 years. Preliminary results show consistency among the Maumee River walleye runs in 1995, 1998, 2005, 2006, 2007, and 2008, with 2003 being significantly different. Similarly, the Sandusky River runs in 1995, 1998, 2005, 2007, and 2008 are congruent and differ from 2003. The 2003 Spawning groups in Van Buren Bay versus the Maumee, Sandusky, and Grand (OH) Rivers showed unusually high inter-run gene flow. The 2003 year class was the most successful in decades, and possible correlation with high gene flow is further examined. Our investigation thus illustrates the importance of testing temporal patterns of genetic variation to understand stock structure

Abstract: Among works, devoted to the quantitative study of style, an approach prevails which can be conventionally called as synchronic. Synchronic approach is aimed at solving various classifi cation problems (including those of attribution), making use of average (mean) values of characteristics, which refl ect the style of the whole creative activity of an author. This approach is based on the assumption that the features of an individual style are not changing during lifetime or vary in time very little, due to which the changes can be disregarded as linguistically irrelevant. This assumption can be tested in experiments, organised within a diachronic approach, whose purpose is to compare linguistic properties of texts, written by the same author at different periods of his life. This paper presents the results of such a diachronic study of the individual style of famous American romantic poet E.A.Poe. The study was aimed at fi nding out whether there were linguistically relevant differences in the style of the poet at various periods of his creative activity and if so, at revealing linguistic markers for the transition from one period to the other.

Abstract: DNA methylation, one of the epigenetic mechanisms, plays an important role in numerous biological functions in cells, from inhibition of cleavage by endonucleases to inhibition of transcription factor binding. The DNA methyltransferase I (DNMT1) protein represents a major DNA methyltransferase activity in human cells and is therefore a prominent target for environmental epigenetic control and experimental cancer therapies. However, there are only few available structural information and inhibitors as well as their high toxicity and low specificity have so far precluded their broad use in chemotherapy. Therefore, cloning and bacterial expression of several functional domains of DNMT1 were carried out for the structure determination by X-ray crystallography and NMR technique. One of the functional domain, DBD (the non-catalytic amino terminus of DNMT 1) binds a new protein, DMAP 1 (for DNMT 1 associated protein), and can mediate transcriptional repression. Bioinformatics, CD (circular dichroism) and NMR (nuclear magnetic resonance) spectroscopic approaches indicate this domain adopt the all helical topology. In addition, DBD showed DNA binding activity, and the responsible sequence was narrowed to about 80 amino acid residues involving the proliferating cell nuclear antigen (PCNA) binding motif. The DNA binding activity did not distinguish between DNA non-methylated and methylated states, but preferred to bind to the minor groove of AT-rich sequence. Furthermore, DNA binding activity of DBD may contributes to the localization of DNMT1 to AT-rich sequence such as satellite, Line 1, and the promoter of tissuespecific silent genes. The structural analysis of DBD of human DNMT1 could provide biological explanation for how the domain interacts with other regulation proteins onto the particular sites in genes expression, and give the structural view for specific inhibitor development.

Abstract: Paralarvae distribution and abundance were studied in the common squid, Loligo vulgaris, by examining 367 specimens collected off the Ria de Vigo (NW Spain, NE Atlantic Ocean) for the period 2003-2005. A total of forty seven surveys?45 and 4 during daylight and at night, respectively- were undertaken to collect plankton and to obtain hydrographic samples. Four transects were studied in the inner and outer sides of the C?es Islands (Ria de Vigo, north-western Spain). Paralarvae were present all year round in this area with a peak of abundance located in spring-early summer. They were more abundant near the bottom at daylight and in surface waters at night. Furthermore, the number of specimens collected at night were much higher (maximum abundance of 43 paralarvae 1000m-3) than those obtained during daylight (maximum abundance of 7 paralarvae 1000m-3). Most of these specimens were captured in the outer part of the R?a. The relationship between chemical oceanographic data and the abundance of the larvae was also assessed.