Background: microRNAs (miRNAs) that play important roles in organisms are associated with usual function of cells, development of living, biological processes as well as many diseases involving cardiovascular disease. miRNAs are effective in endothelium disfunction, atherosclerosis, inflammation, apoptosis and angiogenesis related with coronary artery disease (CAD). miRNAs that are explained differently in cells, exist extracellularly, in circulation and body fluids. Many biomarkers related with CAD can get through circulation and pericard fluid (PF), the closest place to heart. That is why in etiopathogenesis evaluation of CAD miRNAs can be used diagnostically in PF and circulation. Since PF has a potential of reflecting heart potential, PF concentration of some substances provides to be understood many pathophysiological mechanisms. \r\nPurpose: The main purpose of this study is; to determine the amount of miRNA’s in the PF and blood plasma of patients with coronary artery disease (CAD) and to contribute to the understanding of the pathophysiology of CAD. Methods: 40 patients having coronary artery bypass surgery were involved to our study and expression levels of miRNAs (miR-125b-5p, miR-320b, miR-34a-5p, miR-16-5p, miR-320a and miR-15a-5p) that their association with cardiovascular diseases was obtained, were studied in PF and blood plasma with qRT-PCR (Quantitative Real Time PCR) method. Results: In gene expression of miRNAs as gradual change in plasma than PF was found for miR16-5p 7.3539 and for miR320b 4.0715, as a result of this miR16-5p p=0.00001 value and miR320b p=0.00814 value were found statistically significant (p<0.05). Conclusion: The reason of gene expression level of miR 16-5p and miR 320b were found higher in plasma which is expected to be found high in PF may be extramyocardial sources. Obtaining of miR 16-5p and miR 320b in PF that have negative effect in terms of cardiovascular diseases strengthens the potential usage of these microRNAs as biomarker in cardiovascular disease. Although in previous studies it was defined as miRNA special to PF, obtaining of miR320b also in plasma shows that it is not only special to PF.\r\n

Username
Password