Rhodotorula glutinis producing exopolysaccharide, locally isolated was characterized and identified based on morphological and biochemical keys. Rhodotorula glutinis showed folds increase in exopolysaccharide (EPS) production on medium containing glucose or sucrose, respectively.In addition, the synthesis of exopolysaccharide in batch fermentations was improved by adding glucose. Exopolysaccharides synthesized by Rhodotorula glutinis were isolated from the supernatant and identified as D-mannose. Carotenoids produced by Rhodotorula glutinishave been identified as carotene torulene. EPS producing cells were taken to design BENT powderbased formulation. The formulation and storage of a dried biocontrol product was done and the impact of EPs on cell survival during drying and storage was also studied.Biological test showed antioxidant and antifungal trends against blight and spots causing fungal pathogens of Aspergillus flavus, A. niger, Alternaria triticina, Fusarium moniliforme and Penicillium chrysogenum on wheat grain grown under biotic and abiotic stress and significantly enhanced seeds germination, plant growth and wheat grain yield.

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