The aim of this study was to investigate the effect of CBD injection on breast cancer MDA-MB-231 type cells on cell proliferation and cell survival. MDA-MB-231 cells were obtained from the Public University Genome and Stem Cell Institute. CBD was obtained from Yozgat Bozok University Hemp Research Institute. CBD was dissolved in dimethyl sulfoxide (DMSO). Cell cultures were injected with CBD concentrations of 5, 10, 25, 50 and 100 µM. The toxic effect of DMSO on cells in which it was injected in pure form was found to be limited. CBD slowed down the division and proliferation by acting on MDA-MB-231 type cells. The most effective dose was determined to be 10 µM CBD concentration. As a result, CBD slowed down the cell cycle by acting on MDA-MB-231 type cells. According to this study, it can be recommended to investigate CBD concentrations between 6-24 µM in animal experiments.

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